Interestingly, the nuclear export sequence of Mia1p appears to overlap with the Alp14p binding site.
The mutant of the nuclear export sequence (NES) of Ci (L768A) was constructed using a PCR-based method.
The ABL protein contains three nuclear localization signals (NLS) and a leucine-rich nuclear export sequence (NES) [19], [20], [21].
Here we show that Mia1p/Alp7p shuttles in and out of the nucleus during interphase by utilizing a nuclear import sequence (NLS) and a nuclear export sequence (NES).
Upon activation, p38α phosphorylates MK2 at the regulatory sites, a masked nuclear export sequence becomes exposed and the active complex is translocated to the cytoplasm [52].
Once the capability to migrate from the nucleus to the cytosol was assessed, we wondered whether BRMS1 would harbour a nuclear export sequence (NES).
GSK3β phosphorylates both the NES (nuclear export sequence) and the destruction box in Snail and thereby triggers ubiquitin mediated proteasomal degradation of Snail [37].
Considering that Pias proteins bear both a nuclear localization sequence and a nuclear export sequence [15], we speculated that the import of Trim32 into the nuclear dots might be mediated by the dynamic nucleocytosolic shuttling of Pias3.
Subsequently, BRCA1 has been found to posses two nuclear localizations signals (NLS) as well as one nuclear export sequence (NES) that guide the shuttling process of BRCA1 [64], [65], [65].
TDP-43 comprises two RNA recognition motifs (RRMs), a nuclear localization signal and a nuclear export sequence mediating nuclear shuttling, as well as a C-terminal glycine-rich domain (GRD) implicated in TDP-43 protein interactions and functions [1].
A possible consensus nuclear export sequence is LX 1 3 LX 2 3 LXL, where L = leucine and X = any amino acid and the last leucine can be replaced by conservative substitutions (isoleucine, valine, etc).
