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On the other hand, Figure 5A of reference [8] also shows that the nuclear export rate of Smad4 is about half of the nuclear export rate of Smad2.
The nuclear export rate of EGFP-LC3, measured by photobleaching the cytoplasmic pool of the protein, was also markedly slower than that of EGFP (Figure 4B).
The nuclear export rate of a protein depends on the activity of its NES, which in turn is determined by the strength and accessibility to the domain [45].
Tor kinase mediated this effect through negative regulation of the phosphatase Pph21/22 as given by Equation 7. We have analyzed the sensitivity with which TOR pathway regulated the phosphatase Pph21/22 and thereby the export rate of Msn2/4.
Nuclear localization of mRNA does not necessarily imply increased nuclear lifetime, namely, low export rate of mRNA.
We also tested the influence of the import rate of pSTAT1 molecules into the nucleus, and of the export rate of STAT1 from the nucleus into the cytosol.
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During the day, starch is synthesised to serve as an intermediate store of carbon fixed during photosynthesis when rates of production exceed the export rates of the chloroplasts.
Using labeling efficiency and thymic cellularity to normalize the data, we found no difference in the export rates of mature thymocytes when comparing wt and ADAM8−/− mice (Figure 4C).
These data derived from HeLa cells indicate that the nuclear import and export rates of RPL27-GFP are different.
In general, the dynamics underlying the receptor cycle of Pex5p seem to be of importance because the import and export rates of Pex5p have to be balanced [ 55].
The nuclear export rates of Mlxipl and Nlrp6 were also substantially lower than their cytoplasmic degradation rates (cytoplasmic lifetimes were 0.85 ± 0.4 hr for Nlrp6 and 0.18 ± 0.09 hr for Mlxipl).
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