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Then, in the exponential step mode, square waves of 32, 64, 128, 256, and 512 Hz are sequentially outputted and responses are acquired.
Each mark in Fig. 13 represents calculated frequency response by using each source calibration signal (initial frequency = 32 Hz, number of steps = 10, exponential step mode).
By using the minimized number format, we implement core algorithms in seismic applications (complex exponential step in forward continued based migration and 3D convolution in reverse time migration) on FPGA and show speedups ranging from 5 to 7 by including the transfer time to and from the processors.
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If, alternatively, I take 30 exponential steps from the same starting point, I end up a billion metres away, or orbiting the earth 26 times".
With the method described in this study (Fig. 1, Table 2), samples from all three mutant strains showed one or two visible bands larger than 2 kb (Figs. 2a, 3, 4a) after the second exponential amplification step.
Next, exponential smoothing (step 4) is applied, defined by begin{aligned} &text{SF}_{text{s}}(1) = text{SF}(1) ~text{and} &text{SF}_{text{s}}(n) = alpha cdot text{SF}(n) + (1-alpha) cdot text{SF}_{text{s}} n-1) & quadquadquadqquadqquadqquadqquad {for} n-1 2, ldots,L, end{aligned} (4).
Northern blotting is more quantitative than RT-PCR, since there is no exponential amplification step.
In the exponential amplification step, the SuperPrimers are present at a concentration that allows for asymmetric PCR amplification of the biotin-labeled single-stranded DNA targeted by the reverse SuperPrimer.
The recombinant protein was expressed in E. coli JM109 strain during 4 h at 30°C following addition of 1 mM isopropyl- β-D-galactoside during the exponential growth step of the bacterial cell culture.
After the forward primer is depleted in the exponential amplification step, a linear phase is commenced in which preferentially the Cy3-labelled primer is extended, thus increasing the signal compared to an asymmetrical PCR (as performed by [ 23] where only the primer concentrations differed.
Both the variable and constant time step exponential methods of EPIRK-type are used to simulate magnetic reconnection and the Kevin–Helmholtz instability in plasma.
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