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The third step contains one exponential reaction.
The reaction conditions of this exponential reaction are shown in Table 2.
The second step also included two reactions: a linear reaction and an exponential reaction.
The first step includes two reactions:a linear reaction and an exponential reaction.
The product was diluted 100-fold and used as the template for the subsequent exponential reaction.
The reaction conditions for this exponential reaction are shown in Table 2.
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On-line monitoring of the accumulation of amplicons formed in the competitive duplex ARMS in a real-time PCR machine (rtARMS) enabled mtDNA quantification over a wide linear dynamic range (100 1,000,000 mtGE/20µl reaction) and revealed apparent single-cycle PCR efficiencies during the exponential reaction-phase of 98.3% 95%% confidence interval: 96.5 100.2%; 7028C) and 106.4% (101.5 111.8%; 2706G).
A single- or double-exponential reaction trace was observed.
The observed reaction was a single-exponential reaction, and the observed rate constant (kobs) decreased as the hcTn concentration increased until saturation, typically at 2.5 μM hcTn with a kobs of 2.0 s–1.
In this work, a new signal amplified strategy was constructed based on isothermal exponential amplification reaction (EXPAR) and hybridization chain reaction (HCR) generating the hemin/G-quadruplex horseradish peroxidase-mimicking DNAzyme (HRP-mimicking DNAzyme) nanowires as signal output component for the sensitive detection of thrombin (TB).
We have also explored heaters with temperature profiles suitable for other isothermal amplification techniques requiring different incubation temperatures, e.g., the Exponential Amplification Reaction (EXPAR), Nicking Enzyme Amplification Reaction (NEAR), or Recombinase Polymerase Amplification (RPA), could be integrated with this method.
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