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Fig. 5 Exponential feeding strategy with respect to time Fig. 6 Optimal control profile for constant and exponential feeding strategy.

Fermentations with a specific growth rate of 0.25 h-1 were conducted only under exponential feeding.

Conventional exponential feeding strategies indeed fail to account for the loss of biomass induced by the foaming.

The optimal control profiles (constant feeding and exponential feeding strategy with glucose, nitrogen and phosphorous) obtained by using genetic algorithms are generated.

Strain PGLOD4, which contained four copies of the target gene, was chosen for subsequent fermentation experiments, and a fermentation strategy involving two exponential feeding phases was developed.

However, in an effort to avoid nonanoic acid and acrylic acid overfeeding, exponential feeding at 0.15 h-1 was conducted for 23.3 h before changing to a constant feed rate of 8 g L-1 h-1.

The feedback control started functioning at the same time as starting exponential feed rates (see an arrow in Fig. 3a).

Cells were grown exponentially in the bioreactor at a specific growth rate of 0.3 h-1 by using an exponential feed of complex media and induction was done at an OD between 20-25.

Once the initial glucose (15 g/L) was exhausted and the dissolved oxygen spiked, an exponential feed with a 6 hour doubling time was initiated.

Exponential feed of substrate helped to prolong the exponential phase of growth, to reduce the fermentation time and thus the cost.

In this protocol, exponential feed of substrate helped to increase the exponential phase of growth, thereby allowing a high cell density of 37 g L-1.