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To determine the mechanism by which these NSAIDs reduce cell viability, we assayed treated cells for evidence of apoptosis by utilizing Annexin V staining, and explored expression of mediators of apoptosis including caspase activity and levels of Bax and Bcl2.
We also explored expression of Dp71 using immunofluorescence on sections from mouse and rat hippocampus (Figure S2).
Two other reports have explored expression of selected genes by using dedicated arrays in Hfe−/− mice and in mice with secondary iron overload produced by intraperitoneal injection of iron-dextran [19], [20].
Several groups explored expression analysis by hybridizing mRNA to cDNA libraries gridded on nylon filters.
We explored expression patterns at a specific stage of flowering development in an early flowering trifoliate orange mutant by MPSS.
Furthermore, while many microbial studies have explored expression of nutrient transporter genes, few studies have concurrently measured nutrient assimilation rates.
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There it was, open and explored, celebrated in expressions of colour, art and humour.
We were cognizant of this issue; however, as this was one of the first studies that explored sexually dimorphic gene expression changes in human and murine kidneys we opted to maximize the sensitivity at the expense of specificity.
In order to assess the cellular and molecular basis of the metformin-associated decrease in endothelial senescence, we examined SIRT1 expression and explored changes in the downstream signalling pathways for SIRT1 in MMECs treated with HG and metformin for 72 h.
In this study, this expression was further explored in cancer cell lines and immunohistochemically in colon cancer tissues.
These changes are less explored in humans, however, increased expression of the inflammatory macrophage phenotype marker; CD11c in skeletal muscle of T2D patients has been reported.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com