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To further explore the requirement of IKK in efficient DSB repair, we generated stable IKKα and/or IKKβ knockdown cell lines, e.g. IKKα, IKKβ, and IKKα/β cells, by transfection of MCF-7 cells with lentiviral short hairpin RNAs (shRNAs) that specifically target IKKα and/or IKKβ mRNA.
To further explore the requirement of dNF-YA for sev gene promoter activity, dNF-YA RNA interference experiments in S2 cells were carried out (Fig. 6).
Further study is needed to explore the requirement of Smac for full execution of gold III) porphyrin 1a-induced apoptosis in neuroblastoma cells.
To explore the requirement of Caf1 in deadenylation by a trimeric BTG2 Caf1 Ccr4b complex in more detail, we used selective Caf1 inhibitors [ 45].
Additional studies are underway to explore the requirement of ZNF217 in ERα gene regulation at co-bound sites prior to estrogen stimulation.
(50) They confirmed the results of Yu et al. and used AEA as a model substrate to explore the requirement of each enzyme for a free carboxyl group in the substrate.
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To further explore the requirements of dTIEG function, the Minute technique was used to provide a proliferative advantage to mutant cells [30].
To further explore the requirement for the addition of a ribonucleotide cofactor, we measured pol V Mut binding to p/t DNA and DNA synthesis as a function of the concentration of added ATPγS or ATP.
First, we explored the requirement of TLRs in NF-κB induction while the above-mentioned report investigated TLR involvement in the expression of various inflammatory molecules (e.g., TNFα, MCP-1, IL-12).
In the third paper, Hettinger et al. (2015) explore the requirements for, and potential applications of, sociotechnical system models for research as well as in support of the design of safe and productive work systems.
The chapter explores the requirements of the PCI DSS guidance that are related to vulnerability management.
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