In agreement with exploratory activity recorded in the open field, which lasted 10 min, a similar activity profile was observed by the PhenoMaster during the initial exploration period (Fig. 2B).
Fish were further subjected to the novel tank test and their exploratory profile was recorded during a 15-min trial.
Locomotor and exploratory behaviors were recorded with twelve Digiscan open field (OF) apparatus and Versamax software, v.4.00-127E (Accuscan Inc.ruments, InColumbusmbus, OH).
Locomotor and exploratory behaviors were recorded with twelve Digiscan open field (OF) apparatus and Versamax software (Accuscan Instruments, Inc., Columbus, OH).
Locomotor and exploratory behaviors were recorded for each individual mouse for 60 min using eight Digiscan open field (OF) apparatus and Fusion software (Accuscan Instruments, Inc., Columbus, OH).
Exploratory behavior was recorded with a high definition digital camera and on-line videotracking was implemented using custom software written in Matlab environment.
Animals were individually placed in a glass cylinder (diameter, 20 cm) and weight-bearing forepaw contacts with the glass resulting from spontaneous exploratory behaviour were recorded.
Habituation consisted of the acclimation of the animal to the arena for 5 min, during which the number of lines each animal crossed on the floor of the arena (number of crossings) and the number of rearings (elevation on rear paws, denoting exploratory behavior) were recorded using manual stopwatches to verify locomotor/exploratory activities.
Moreover, Blackstar, apparently created in the knowledge he did not have long left and released two days before he died, is a very good record indeed: exploratory, unsettling, the work of someone still minded to take inspired risks.
More information on the recording and quantification of exploratory behavior (e.g., video recording, tracking, and interrater reliability) would be helpful for assessment and replication.
