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We thank A. Buchberger, H. Rao, A. van Hoof, R. Voorhees and J. Warner for reagents; the Proteome Exploration Laboratory (PEL), Caltech, for help with the mass spectrometry analysis and the members of the Deshaies laboratory, M. Blanco, M. Guttman, B. Clemons, R. Voorhees and S. Shan for discussions.
Two sets of replicate libraries were prepared and stored in separate −80°C freezer banks at the Mississippi Genome Exploration Laboratory (MGEL; www.mgel.msstate.edu) while the original was sent to the Clemson University Genomics Institute (CUGI; www.genome.clemson.edu) for distribution and remote storage.
We thank the Caltech Proteome Exploration Laboratory for mass spectrometry expertise.
Copies of the BAC library, individual clones, and macroarrays can be obtained from the Mississippi Genome Exploration Laboratory [ 14].
Blood was suspended in citrate buffer (250 mM sucrose, 40 mM trisodium citrate, pH 7.6) containing 5% v/v dimethylsulfoxide, aliquoted into 1.5 ml polypropylene tubes, flash frozen in liquid nitrogen, and shipped to the Mississippi Genome Exploration Laboratory [ 14].
We would also like to acknowledge Dr Dan Peterson at Mississippi Genome Exploration Laboratory (MGEL), Mississippi State University, USA, for providing the BAC library resources, and to thank both him and Dr Xueyan Shan for their contribution to the project.
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