We used the following variables as explanatory variables: expression abundance, gene body methylation, promoter methylation, and gene lengths.
In fact, either haploid fitness or synthetic lethal interactions alone are enough to render the explanatory power of expression level and protein-protein interactions insignificant (p>0.18 in each case).
Participant explanatory statements and expression of interest forms were also made available.
The explanatory variables were gene expression levels, expressed as arbitrary units of fluorescence intensity, for a set of 60 candidate genes selected based on potential for involvement in fire blight susceptibility.
Interestingly, Anduril-based analysis of the two most frequently deleted genes at 9p21.3, MTAP and CDKN2A, shows that even though the gene deletion is an explanatory factor for lower expression of these genes in patients with deletion, in total GBM patient material the MTAP expression is not inhibited and CDKN2A is overexpressed compared to normal tissue (Additional file 4).
Tree size, age and competition within forest stands were found to be the dominant explanatory variables, whereas the expression of site characteristics, environmental and climatic drives was weaker.
Since coping styles have been found in many animal species, this is of considerable general significance; we conclude that similar beneficial effects of including coping style as an explanatory variable in gene expression studies are likely to be widespread.
A single F-statistic p-value was determined to evaluate the explanatory value of the expression data using all days.
Despite the striking concordance between DNA methylation and hPTMs, however, our results suggest the function of DNA methylation is not entirely redundant to hPTMs DNA methylation retains explanatory power for gene expression levels when controlling for numerous hPTMs.
The most important explanatory variable of gene expression profile variance across samples in our dataset was in fact not the platform on which the RNA was assayed, but how the tissue was preserved.
We used seven histone methylation levels (H3K4me1, H3K4me2, H3K4me3, H3K9me3, H3K20me3, H3K27me3, H3K36me3) in mouse embryonic stem cells [ 24, 32] as the explanatory variables and gene expression levels as the response variable.
