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Pairwise Fst and Rst statistics were used to compare population genetic models explaining divergence among subpopulations (step-wise mutation vs geneflow or drift) based on nuclear microsatellite markers (amplicon length) (Slatkin [1995]; Hardy et al. [2003]) using Arlequin v3.5 (Excoffier and Lischer [2010]).
Duplication induced mutations explaining divergence; and recombination harbored new folds of new proteins with new functions that evolved from common ancestors [ 40, 46].
The geography of the island has historically been considered to play a significant role in explaining divergence patterns among lemurs and other vertebrate species (e.g. [ 26, 28- 32]) and specifically Microcebus species [ 33- 36].
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We suggest that a constraint early/opportunity late model best explains divergence over ontogeny.
In addition, allopatric divergence in rainforest refugia does not seem a likely mechanism to explain divergence of the lowland tapir as the species' distribution ranges across South America [ 13] including large areas of savanna [ 31].
Specifically, we observed strong additive effects for each trait measured, and a simple additive model was sufficient to explain divergence in important body size attributes (e.g., snout-vent length, mass, growth).
If the fitness landscape is similarly rugged within G. manniand Gambusiasp, then the latter two mechanisms could explain divergence in the face of similar selection – because multiple adaptive solutions to environmental constraints on signal transmission exist.
Other isolating mechanisms are appropriate to explore in this system to help explain divergence patterns not well supported by vicariance, such as the effects of rapidly evolving gamete recognition proteins on isolating populations.
Covarion models are useful not only because of improved phylogenetic estimation; they can also be used to identify patterns of sequence evolution that explain divergence in protein function or structure.
In cases where distance does not explain divergence, it has been suggested that circulation patterns, such as recirculation in a coastal fjord, may provide a sufficient barrier to gene flow, allowing populations to diverge and perhaps adapt to local conditions (Rynearson and Armbrust 2004).
As more species are shown to vary in sensory systems across populations (e.g. Bluefin killifish [ 43], cichlids [ 57], stickleback [ 58], sand goby [ 59], pied flycatchers [ 60], guppies [ 12]), Sensory Bias models become a more likely candidate to explain divergence in mate preferences across species.
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