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The experiments were validated using 750 egg surface images with 94% accuracy and 1.67% false negatives.
Microarray experiments were validated using qRT-PCR as previously described (Posnack et al. 2011).
A set of genes altered in microarray experiments were validated using a second method like semi quantitative RT-PCR.
A subset of results from the microarray experiments were validated using quantitative real-time polymerase chain reaction as described in File S1, Figure S2, Figure S3, Table S5, Table S6, and Table S7.
Some experiments were validated using Fura-2, a ratiometric dye, to take into account some of the disadvantages of using a single wave dye such as Fluo-4 AM.
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Transcription dynamics observed in the microarray experiments was validated using quantitative real time PCR (qRT-PCR) for a selected subset of up and down-regulated genes.
The performance of each capillary on the four DNA analyzers used in this experiment were validated using one 384-well control plate for each instrument.
These correlations were validated using experiments conducted for horizontal hydrogen jets in the vicinity of horizontal surfaces, carried out at the facilities of Defence Research and Development Canada (DRDC) in Val-Belair, Québec.
The reliability and accuracy of the models were validated using experiment and finite element analysis results.
Finally, the results were validated using confirmation experiments.
Pathogenicity was assessed using a bioinformatics pipeline and novel variants were validated using functional experiments.
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