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While en route most of the scientific instruments and experiments were tested and calibrated.
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Specificity of the antibodies in immunolabeling experiments was tested and confirmed on samples from Kv4.2−/− mice.
The weights and compositions, etc. of the specimens before and after corrosion experiment were tested and analyzed, the microstructures of the specimens were also inspected by SEM.
Relative expression ratios from RT-qPCR experiments were tested for normality and equal variance and log transformed to allow assumptions to be satisfied before being subjected to one way ANOVA using Minitab 16.1 software (Minitab Inc., UK).
All experiments were tested in three replicates and randomized to minimize the effects of unexpected effects in the observed response.
The proposed experiments were tested on human α-synuclein and ubiquitin, two representative examples of unfolded and folded proteins.
All experiments were tested for statistical significance using ANOVA and a P⩽0.05 was considered significant.
Before and during the experiment, these new devices were tested and debugged, and their performance was also evaluated.
Three replicates were tested and experiments were conducted three times.
Instead, the greatest problem is in simply describing what the data represent: what experiment was done, what hypotheses were tested, and what ancillary sample parameters describe the data.
Eleven transcripts identified as differentially expressed in the microarray experiment were tested using sqRT-PCR (Table 4 and Additional file 7).
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