Great tufts of mugwort, an ancient beer ingredient, which the owners scavenged from Prospect Park for a brewing experiment, are suspended from a high ceiling, next to light fixtures made of bare bulbs inside mason glass.
This experiment was suspended, but not forgotten, after 11 March 2011.
In all experiments cells were suspended in the growth medium and seeded in 24-well plates at 2 × 105 cells/well.
For UV radiation experiments, cells were suspended in phosphate-buffered saline (PBS) and exposed for 20 min to 1.5 kJ/m of UVB radiation (290 320 nm) and incubated at 37°C in fresh culture media for 4 h.
For these experiments, cells were suspended in respiration buffer (1 × 10 cells/mL) and digitonin concentration was titrated in intact cells by respirometry in the presence of 0.5 mM rotenone (to inhibit endogenous respiration of intact cells), 10 mM succinate, and 1 mM ADP.
During the experiment, tiles were suspended in separate containers (1 L).
One day before the experiment, cells were suspended in 200 μl of medium in a 96-well plate at a concentration of 5000 cells per well.
VSMCs subjected to adenovirus infection in the absence or presence of AngII according to the experiment requirements were suspended in DMEM/Ham's F12 containing 0.5% FBS.
In in vivo experiments, these agents were suspended in 0.5 w/v% methylcellulose 400cP solution (Wako Pure Chemical Industries, Osaka, Osaka, Japan) to make dosing formulations.
