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Three independent DamID experiments were performed utilising biological triplicates in first two studies and quadruplicates in the third study (Supplementary file 2C).
To determine the ability of PEX5 to form complexes with PEX14N and PTS1 cargo, pull-down experiments were performed utilising purified soluble recombinant constructs (Figs. S1 and S2) and a fluorescently labelled PTS1 peptide.
To expand this finding, a third set of experiments were performed utilising diltiazem as a tool to alter Ca2+ transient parameters to a similar degree as reducing [Ca2+]o alone.
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To isolate the ternary complex, a pull-down experiment was performed utilising the StrepII tag of the soluble PEX14N construct (Fig. S1).
Monte Carlo simulations were performed utilising a Python script.
All analyses were performed utilising Statistical Package for Social Sciences 15.0.
Bioinformatic analyses were performed utilising the MiSeq Reporter Software MCS 2.2.0, RTA 1·17·28·0 and Nextgene (from Biogene, Kimbolton, Cambs, UK).
All the statistical procedures were performed utilising the SPSS 19.0 software for Windows (SPSS Inc., Chicago, USA).
Analysis and graphing were performed utilising Sigmaplot 8.0 (SPSS Science Marketing Dept. Chicago, IL, USA).
Experiments will be performed utilising Coulomb excitation, inelastic-scattering, transfer and fusion evaporation reactions using stable and radioactive ion beams with suitable heavy-ion detection.
Statistical analysis was performed utilising GNU R [29].
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