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Immunoblotting experiments were performed using established methods.
HS-AFM experiments were performed using a laboratory-built instrument57.
Experiments were performed using cells from five cattle.
SPR experiments were performed using a Biacore X biosensor (GE Healthcare).
Experiments were performed using three biological replicates of culture and transfection conditions.
Experiments were performed using a Ag/AgCl reference (ΔEref=±1 mV).
Blinded time series experiments were performed using images recorded continuously from a single focal plane.
The experiments were performed using a Zeiss 55VP System (Oberkochen, Germany).
For ARE-Luciferase assays, experiments were performed using the calcium phosphate transfection method.
Statistical analysis for the in vitro experiments were performed using the unpaired Student's t-test.
Electrophysiological experiments were performed using a PC2C patch clamp amplifier with corresponding software (InBio, China).
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