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The solubility and diffusivity of carbon dioxide in aqueous TREA solutions which are needed to interpret the kinetics experiments were determined using the nitrous oxide analogy method.
Protein concentrations for immobilization experiments were determined using the bicinchoninic acid assay (Pierce ™ BCA Protein Assay Kit Thermo Fisher Scientific, Darmstadt, Germany) according to the manufacturer's protocol.
The size and density of the catalyst droplets resulting from the Au layer annealing experiments were determined using the image processing software "ImageJ" [19].
Statistical differences of all the " in vivo" experiments were determined using chi-square test.
The stimulus parameters used for our experiments were determined using control flies (w 1118 ; UAS-TNT; pBPGAL4U).
Statistical differences in the proportion mice with lung metastases in xenograft experiments were determined using Fisher's exact tests (two-sided).
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The reproducibility of the experiments was determined using commercially available BMSCs (StemPro Rat Alkali Phosphatase-expressing Mesenchymal Stem Cell, Invitrogen).
A significant correlation coefficient of 0.22 (p<0.0001) between the two distinct microarray experiments was determined using the scored T-test for the most differentially expressed genes in both platforms (p<0.05).
Statistical significance in quantitative PCR experiments was determined using a one-sample Student's t-test.
Significance of lifespan experiments was determined using the log rank test (Lawless, 1982).
Statistical significance of experiments was determined using Student's t test, with a significance level of p < 0.05.
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