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Afterwards, the tissue was perfused with aCSF-HEPES for at least 20 min to allow for de-esterification of the dye before imaging experiments were commenced.
The animals were allowed between 7 10 days to recover from surgery before experiments were commenced.
Experiments were commenced 7 10 days after surgery.
PCOS induction experiments were commenced at ∼28 days.
An equilibration time of 15 min was used before experiments were commenced.
All experiments were commenced only when the mortality rate of zebrafish was <2% for at least one week.
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Jatropha curcas (KUBP 78-9 Var)., having been planted at 2 × 2 m spacing, was aged 2 yr when the experiment was commenced.
The experiment was commenced at 6 a.m. of a day and those were completed at 6 a.m. of the next day to observe the daily feeding rate.
Before the formal experiment was commenced, the subject went through sufficient training so that the thresholds could be stabilized and proper intervals for the 7 orientations of the test line could be empirically determined.
In the anisomycin experiment, recordings at 15-min intervals were commenced 15 min after injection.
Large projects were commenced.
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CEO of Professional Science Editing for Scientists @ prosciediting.com