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Data from interviews and walking experiments were collected to validate the model.
Trophozoites from feeding or soaking experiments were collected to prepare crude extracts using a protease inhibitor cocktail (Roche) as previously described [12].
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Further, the algal cells from certain treatments of this experiment were collected to directly visualize whether Ag-ENs can enter the cells with TEM and Z-contrast dark-field scanning transmission electron microscope (STEM) following a similar procedure described by Han et al. [15].
Culture samples for microarray experiments were collected at exponential, transition, and stationary phases.
However, for V22-SH3, NMR HSQC experiments were collected at both pH 4.0 and 6.2 for comparison.
At the end of both experiments, blood samples were collected to determine plasma leptin and insulin concentrations.
At the end of experiment, blood, liver and brain samples were collected to assess biochemical and biophysical markers as well histopathological investigations.
Culture medium from triplicate wells from three tissue biopsies (n = 3 separate decidualization experiments) were collected, pooled and concentrated 5-fold to measure BMP2 (ELISA; R&D Systems), BMP4, BMP7, TGFβ1 and TGFβ2 (ELISA; Ray BioTech., Norcross, GA, USA) according to the manufacturer's instructions.
Lawn cuttings for experiments were collected from different places.
METHODS: Data from more than 3,250 large animal experiments were collected from studies focusing on the effects of blast exposure.
All samples and experiments were collected and made in France.
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