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In this study, samples used in experiments were collected by opening a trial pit from the sub-tropical areas of Iran.
Images within each set of experiments were collected by using identical settings for pinhole diameter, laser power, detector gain, and offset.
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DNA fragments generated from the GP experiment were collected by excising their bands from polyacrylamide gels, the most reliable method for obtaining sequences common to both GP and conventional sequencing.
The majority of the data used for this experiment was collected by Tang et al. [40].
The sequences of differentially expressed genes identified by the microarray experiments were collected from NCBI GeneBank [ 39] and compared them to known sequences from Cotton Gene Index [ 40] using the Basic Local Alignment Search Tool (BLAST) to determine if there was any significant homology to known gene products.
For subsequent experiments, cells were collected by trypsinisation, counted, and plated as specified below.
In some experiments, mammospheres were collected by centrifugation and dissociated with 0.05% trypsin for 15 min to obtain single-cell suspensions (Liao et al, 2007).
The choice experiment data were collected by randomly interviewing local residents face to face in the urban area of Can Tho City, representing the biggest city in the Mekong Delta and the urban region of Kien Giang Province where U Minh Thuong National Park is located.
Briefly, at the end of the experiment sperm were collected by making ten incisions in the cauda epididymis with a scalpel blade into 200 μL BWW medium [ 73].
For some experiments, cumulus cells were collected by mouth pipetting the COCs repeatedly.
Data from at least three independent experiments was collected, corrected by Renilla activity and expressed as relative light units (RLU).
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