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Q-RT-PCR data from at least three independent experiments were calculated with Delta Ct Method.
The P values for the experiments were calculated with a two-tailed Student's t-test.
P values for statistical differences found in MALDI-MSI experiments were calculated with one-way analysis of variance (ANOVA) with a Bonferroni post hoc test using SPSS SPSS Inc. Chicago ILL, USA) following normality testing.
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Mean±S.D. of at least three independent experiments was calculated with reference to untreated controls defined in a 1.0 scale.
Curve progression of cell growth in cetuximab experiments was calculated with SPSS version 13·0.1 (Apache Software Foundation, Forest Hill, MD, USA).
To verify this possibility a substitution matrix for HisKa exchange experiments was calculated with the Phylip algorithm including sequences from different strains of E. coli, S. typhimurum, B. subtilis and S. aureus (Fig. 1 with detailed coloring).
Additionally, the amount of CO2 released during the incubation time of 14 days of the experiment was calculated with the equation: {D}_t={displaystyle sum}left({V}_0-Vright cdot 2.2 (2).
For validation of remaining SNVs, the allele fraction (AF) from the target capture experiment was calculated with a custom Python script (available at https://github.com/Molmed/Berglund-Lindqvist-2013 //github.com/Molmed/Berglund-Lindqvist-2013 //github.com/Molmed/Berglund-Lindqvist-2013
The miR-221 expression in FFPE experiment was calculated with the formula 2-Δcq, and the change ratio of miR-221 in the in vitro experiments was: (1-1/2ΔΔCq) × 100% [ 62].
Energies involved in collision and abrasion experiments were calculated and showed an exponential correlation with ash production rate.
Values of Reynolds number between 5 and 13 were calculated with numerical experiments based on the Navier Stokes equations (Couland et al. 1986; Stark 1972).
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