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The optimization of experiments was validated and optimum formulation was passed the stability study.
Transcription dynamics observed in the microarray experiments was validated using quantitative real time PCR (qRT-PCR) for a selected subset of up and down-regulated genes.
The increase in CYP1A1 mRNA detected by the microarray experiments was validated by quantitative PCR using CYP1A2 as a negative control (data not shown).
The repeatability of four parallel reactions in one round and three experiments was validated by examining the standard deviation (SD) and relative standard deviation (RSD).
The expression of differentially expressed genes from microarray experiments was validated by qPCR using hydrolysis probe-based inventoried and custom designed PrimeTime qPCR 5′ Nuclease assays procured from Integrated DNA Technologies, Coralville, IA, USA.
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Notably, the results obtained by our experiments were validated by the actual beekeeping reality.
The experiments were validated using 750 egg surface images with 94% accuracy and 1.67% false negatives.
The methylation-specific PCR experiments were validated by DNA sequencing.
Main observations of the microarray experiments were validated by quantitative real-time PCR (qPCR).
Microarray experiments were validated using qRT-PCR as previously described (Posnack et al. 2011).
Results from p38 inhibitor studies in pooled experiments were validated separately at the single cell level as described in Results.
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