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Central composite design of experiments was utilized to determine the optimal pressure and number of cycles of HPS for the highest CNF yield, crystallinity, and lowest CNF diameter.
Design of Experiments was utilized to determine if deposition parameters including the bias-voltage, heating power, and chamber pressure have a significant influence regarding critical scratch load, hardness to Young's modulus ratio, and electrical resistance.
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Real-time PCR experiments were utilized to determine whether the in-vitro-identified effector molecules (aminoacetone and methylglyoxal) as well as the non-effector (animopropan-2-ol) were capable of affecting the expression of the genes in the operon (bxe_B3016, bxe_B3017, bxe_B3018 and bxe_B3019).
Design of experiment (DOE) was utilized to determine the ranks and interactions between each factor from the most influential on the results to the least affects towards the results.
In this study, a total superoxide dismutase (T-SOD) assay kit (Nanjing Jiancheng, China) was utilized to determine SOD activity in the supernatant and experiment was performed following the manufacturer's protocol.
ImageJ software was utilized to determine nanoparticle yields.
A student's t-test was utilized to determine whether two populations were significantly different.
The Bradford protein assay was utilized to determine microbial growth [32].
Linear regression was utilized to determine the end-point titers of the antibodies against different antigens.
In addition, immunoblot analysis was utilized to determine autophagy induction by expression of LC3 II.
Kolmogorov-Smirnov test was utilized to determine data normality.
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