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Finite Element Modeling (FEM) for the blast loading experiments was performed using the Ls-Dyna code.
Process parameter selection for the experiments was performed using Taguchi L9 orthogonal design and measurements of surface were carried out using commercial gloss meter.
The design of experiments was performed using a full factorial method including 31 × 22 × 91 = 108 experiments, to determine the main effects, binary and ternary interactions of variables.
Furthermore, the design of experiments was performed using 5-level-4 factor central composite design coupled with response surface methodology (RS M in order to optimize the transesterification conditions.
Detailed chemical kinetic modeling of the experiments was performed using an updated and improved kinetic scheme (877 reversible reactions and 122 species).
A series of experiments was performed using a set of two diagonally located columns (connected in series) each consisting of five coiled layers of 1 mm I.D. with a total capacity of 27.0 mL.
The design of experiments was performed using a double 5-level-4-factor central composite design coupled with response surface methodology in order to study the effect of factors on the yield of biodiesel and optimizing the reaction conditions.
All set of experiments was performed using ultra pure MilliQ H2O (resistivity: 18.2 MΩ cm).
The entire set of experiments was performed using freshly subcultured strains.
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Immunoblotting experiments were performed using established methods.
SPR experiments were performed using a Biacore X biosensor (GE Healthcare).
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