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Confirmation that the monolayers were stable during the time frame of these experiments was established by performing thiol desorption studies (Fig. S7, ESI †).
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Else, an adaptive refinement of the design of experiments is established by adding optimally selected new support points for informing the metamodel development, and the next SAO cycle remains in the current sub-region.
Background noise for the experiment was established by measuring the laser light that was reflected back from the array in the absence of the phages.
Thus, the violation of expectancy in this experiment was established by the period of exposure (lasting for 30 min) prior to testing.
A protocol for the red cell permeabilization (by hypotonic shock) experiments was established where the conditions of the modifications were controlled in such a manner so that the resulting concentration of the encapsulated hemoglobin could be manipulated as desired.
In a series of column experiments, residual hexadecane saturation was established by pumping 14C-hexadecane into water-saturated sand columns and then flushing with water at a velocity of 25 cm h−1.
In this and subsequent experiments, specificity of binding was established by competition with free 20(S -OHC.
For in vivo experiments, the lung tumor model was established by subcutaneous inoculation of cell suspension on mice, liposomes and free VB were injected i.v. in mice, followed by exposure the tumors to hyperthermia (HT) for 30 min after administration.
For the different experiments, concentration of viable Sc was established by methylene blue exclusion (0.3 g/L methylene blue, 20 g/L sodium citrate).
This was established by experiments involving targeted mutation of promoter binding sites, chromatin immunoprecipitations and inhibition of histone deacetylases.
A direct role for dysfunction of this pathway in cancer was established by experiments in transgenic mice that showed that activation of the Wnt signaling pathway in epidermal stem cells leads to epithelial cancers [ 52].
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