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Control experiments to optimize biophysical related parameters e.g., labeling density optimization, label size, and label specificity.
The optimal experimental conditions were carried out on three repeated experiments to optimize and verify the reliability of the conditions used, and the levels of the other factors were as follows: sonication time was 60 min, enzymolysis temperature was 45 °C, enzyme concentration was 70 mg/g, enzymatic hydrolysis time was 2 h and the crushed mesh number was 0.355 0.85 mm.
A multi-criteria optimization was also carried out by means of design of experiments to optimize remaining parameters: the extraction temperature was set at 40 °C, the ionic strength at 1.77 mol l−1 and the volume of the aqueous solution at 7.2 ml.
Experiments to optimize activities of MST crRNAs with dLbCpf1-VPR.
To preclude false identification of genes as essential due to low transformation frequencies, we performed experiments to optimize the transformation efficiency of S. sanguinis SK3626.
Therefore, we used a fractional factorial design of experiments to optimize the microfluidic, ethylene carbonate buffer-based FISH protocol.
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These requirements cause to apply the technique of experiment to optimize mentioned properties.
First, we performed a time course experiment to optimize the time window for the chemical screen and determined that cell apoptosis significantly increases from day 25 to day 29 of the differentiation protocol (Supplementary Fig. 6a).
The Austre Brøggerbreen and Edithbreen were considered as test sites to setup the experiment, to optimize the sampling strategy and to identify some basic experimental artefacts.
Designing and building a liquid scintillator multiplicity counter (LSMC) requires a symbiotic effort of simulation and experiment to optimize performance and mitigate hardware costs in the final product.
Prior to the coating work, the nanopowders were subjected to a two-level factorial design of experiment to optimize the operational spray parameters, namely, the primary gas pressure, the carrier gas pressure, and the powder feed rate.
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