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Both kinds of problems may necessitate extensive functional experiments to determine which mutations actually cause the selected phenotype.
Therefore, we performed a series of experiments to determine which SNARE proteins (e.g. syntaxin and VAMP) interacted with SNAP 23 upon capacitation.
Each mutant strain may contain 50 100 random mutations, necessitating extensive functional experiments to determine which one causes the selected phenotype.
Prior to the screen we conducted a series of control experiments to determine which Rho allele and what conditions were best for conducting the large-scale screen (details are presented in Materials and Methods).
As the Rac1 inhibitor NSC23766 blocks both Tiam1 and Trio, we performed additional experiments to determine which Rac1-GEF was involved in suppressing BMP-2-induced osteoblastic differentiation.
We screened the 42 experiments to determine which cytokine treatments induced a set of genes that overlapped best with the 709 epidermal PP-increased DEGs.
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Charles Zimmerman, vice president for prototypical design-construction standards at Wal-Mart, said his company was experimenting to determine which technologies provided the most energy savings, because energy was the company's second-biggest operating expense, after personnel.
Lauduree is conducting an elaborate experiment to determine which trees consume the most carbon dioxide and balks at accompanying Greta and Ed because the project can't be completed in a more tropical climate.
A second extension would be to run a richer cross validation experiment to determine which amongst a larger family of statistical models fit the data (and so quantify replication) best.
The goal of the experiments was to determine which distributional semantic model work best for care episode retrieval, and what the best way of calculating care episode similarity is.
This permits, for example, to map high-throughput data coming from transcriptomics or proteomics experiments and to determine which elements of the pathway are affected in a given condition/tissue/organism. Measurements can be coupled with graphical hints so that when the pathway is converted to graphical representation, elements with experimental measurements will be colored accordingly (Fig. 1).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com