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For these experiments, to decrease the possibility of out-of-focus protrusions, we manually monitored the focus of live cells.
As requested, we performed antagomir experiments to decrease the expression of miR-100 in donor cells (new Figure 6D).
Similar experiments were performed using a third expression vector carrying one of the known miR-96 mutations (+13G>A), previously shown, by Northern blot experiments, to decrease the level of miR-96 (14).
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This has been shown in psychological experiments to decrease prejudice.[24].[24]
For the experiments at 35° and 42°C, a sequential design of experiments was used to decrease the number of experiments required to determine the parameters [ 37].
Experiments were duplicated to decrease the errors.
The dilute acid/Fe2+ ion cocatalyst-impregnated feedstock was prepared the same day as the pretreatment experiments were conducted to decrease the likelihood of oxidation of Fe2+ to Fe3+, and the impregnated feedstock was kept under argon.
The reason for this jump is that in real experiments, it is difficult to decrease the voltage immediately, just as it is difficult to decrease the current immediately.
In this work, we established Taguchi's design of experiment as a statistical tool to decrease the amount of experiments, providing the practical optimal condition to operate a chemical sensor with high sensing quality.
A heating pad (Operatherm 200W, KanMed, Bromma, Sweden) was set to 38 °C throughout the experiment to decrease heat losses.
Non-ventilated control mice were selected for blood gas analysis at baseline (for both strains n = 6): animals were handled one week before the experiment to decrease stress activation.
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