Exact(6)
Through a series of experiments, the viability of sonication for pollution control in higher complexity systems was investigated.
From in vitro experiments, the viability of VSMC cells was determined to be 60%and95%5% after 1 and 6 days after seeding, respectively.
In some experiments the viability marker LIVE/DEAD® Aqua (Molecular Probes) was added to exclude dead cells.
In preliminary experiments, the viability of urothelial cell sheets was tested by transfer onto a de-epithelialised stroma and maintenance in organotypic culture [7].
In order to confirm that the tumor samples were still viable during the entire experiments, the viability of the tumor explants was determined at different times before testing the antineoplastic effect of CA, UA, and RA.
In cellular experiments, the viability of longitudinal layer myocytes from guinea pig ileum was not altered in the presence of XF-OE and the Fluo-4-associated fluorescence intensity in these intestinal myocytes stimulated by histamine was reduced by the essential oil, indicating a [Ca2+]c reduction.
Similar(54)
In each experiment, the viability of 20 eggs per group (Candida or PBS control) was evaluated daily by candling for 7 days.
At the beginning of each experiment, the viability and phosphatidylserine externalisation of the aged neutrophils was measured using propidium iodide and annexin V-FITC, respectively, by flow cytometry using a FACS Canto (Becton Dickinson, Oxford, UK).
In some experiment, the viability and the numbers of tumor cells were determined using microscope counts in the presence of a trypan blue dye using tumor cells harvested after treatment with TripLE Select solution (Invitrogen) on day 3 of culture.
In all experiments with inhibitors, a tested concentration was used after careful titration experiments assessing the viability of the macrophages.
Further, in experiments with D-mannosamine, a tested concentration was chosen after careful titration experiments assessing the viability (CFU) of M. bovis BCG.
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