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In the experiments, the population size was 10,000, and each run lasted for 200 iterations.
However, it should be noted that in these experiments, the population grown with AnTc has been enriched for cells that lack the amplification, and thus normalization understates the effect of AnTc on revertant number.
When creating a high titer ΦX174 stock for use in the extended thermal selection experiments, the population incorporated two nonsynonymous mutations (in the F and H coding region) resulting in a modified strain, which we refer to as JACS, serving as the ancestral population of the extended thermal selection experiments (see Methods).
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In this experiment, the population size was fixed to 500 with 1% mutation probability.
Thus, if the quality and abundance are initially low at the start of a microbial culturing experiment, the population focuses on increasing its quality, thereby producing a lag phase in its growth in abundance until the abundance variable crosses a threshold.
During the 32-day experiment, the population numbers peaked at approximately 300 individuals after 28 days in the uninfected populations fed with the P-sufficient algae.
Consequently, at the end of the experiment, the population rate of increase was higher in uranium than in salt and intermediate in the alternating environment.
Consistent with the importance of PEP in this experiment, the population that evolved the BoxG18A allele later also substituted an IS 150 insertion into pykF, which encodes an enzyme catalyzing the conversion of PEP to pyruvate [ 25].
At the end of the experiment, the population rate of increase for uranium treatment reached about 75% of the control rate – in contrast it stabilized at around 35% for the alternating treatments and < 15% in the salt treatments.
In the current experiment, the population was grown for two coppice cycles and therefore QTL mapped for the CC2-4 dand and common to other years are good targets for genotype improvement in this genetic cross.
Most of our results could be validated by in vitro experiments on the population level.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com