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Prior to the experiments, the light output of the optical fiber was adjusted to ~10 mW, measured by an optical power meter.
In the majority of experiments the light scattering was recorded at 297 or 350 nm.
For temperature-controlled experiments, the light scattering instrument was equipped with a thermostat from Julabo.
In all imaging experiments, the light power used at the eye pupil was ~1.8mW, within the safe ocular exposure limits recommended by the American National Standards Institute (ANSI) [ 50].
For the latter experiments, the light intensity (power density) at the sample surface was measured with a spectroradiometer (Solatell (Stroud, UK), Sola Scope 2000TM, measuring range from 230 to 470 nm).
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In most experiments, the lights were turned on continuously and volunteers had no control over the light-dark cycle (except by closing their eyes during sleep).
For light sources of the light induction experiments, the light-emitting diodes (STICK LED, Tokyo Rikakikai) were used for blue light (λmax = 470 nm) and the red-emitting fluorescent tubes FL20S-Re-66 FL20S-Re-66 FL20S-Re-66chnology) filToshiba Lighting & Technologyheet (Acrylite102, Mitsubishi Rayon) filteredlighthrough = 660 nm).
Thus, in this experiment, the light intensity index varies around its optimal value 35, i.e., from 30 to 40 at a step size of 1, while the lens magnification is fixed to its optimal value 3.
In our experiment, the light source was orthogonal, therefore plant orientation did not fully return to the vertical.
In the first SILAC experiment, the light amino acid-labeled cells were treated with dox to reduce the Hsp90 level and the heavy amino acid-labeled cells served as a wild-type control.
In the high-tech, Fantasia-esque video below he experiments with the Light Frequency Fingertips (LFFs) using several mobile phones and a flashing bicycle light.
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