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In "Solution methodology" and "Numerical experiments", solution methodology and conducted experimental results are presented, respectively.
In addition to surface capture kinetic experiments, solution binding studies were also conducted at room temperature (25°C) using a KinExA 3000 instrument (Sapidyne Instruments, Boise, ID) to quantify the equilibrium binding constants of VEGF inhibitors in solution, using varying concentrations of VEGF-A165,VEGF-A121, hPlGF-2 or VEGF-B(10-108).
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They were "imagineers" - designers of gadgets, experiments, solutions.
For these experiments solutions were bubbled with argon before use.
During experiments, solutions in both chambers were continuously bubbled with air.
(In our experiments, solutions with the last property have always been found within milliseconds).
In reduction experiments, solutions were made anaerobic by flushing the cuvette with argon.
For encapsulation experiments solutions of amelogenin was prepared by dissolving lyophilized amelogenin in 0.05% HAc.
During all experiments, solutions were continuously bubbled using 95%/5%O2/CO22 resulting in pH ∼7.4 for control solutions and ∼6.7 for solutions causing metabolic acidification.
Dissolution-DNP experiments: Solutions of 10 m m BT-GGR in the glass-forming mixture H2O/D2O/[D6]DMSO (v/ v/ v=5:35:60) were doped with 25 m m TEMPOL (Sigma Aldrich).
During the experiment, solution was heated and cooled using a Peltier element.
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