As for our subsequent in vivo experiments (see below) the light-inhibition of kinase activity was most valuable, these latter constructs have not been further characterized.
We used experimental containers as described for our main experiments (see below) but did not introduce D. esakii, but only G. affinis and some floating vegetation.
For the 1H, COSY, HMQC, and HMBC experiments, (see below) a three channel 5 mm inverse detection, three axis gradient, variable temperature probe with 2H lock at 76.773 MHz was used.
However, wide bandwith and low anharmonicity of the amplifiers that generate the waveforms turn out to be crucial for shutting down the electric field rapidly and accurately for the imaging experiments (see below).
This hypothesis was tested in subsequent experiments (see below).
Both wild-caught and lab-reared individuals were used for both experiments (see below).
In the scheduled feeding experiments (see below) a cocktail of NMDA antagonists was used.
Five days post-fertilization, planula larvae were put into bottles or cups for the following experiments (see below).
Although an increase in basal expression was also observed for Egr-1, this was not reproduced in other experiments (see below).
The neural retina attached to the epithelium-sclera was used for electrical recording experiments and the isolated neural retina was used for cut-loading experiments (see below).
As in our behavioral experiments (see below), the location of the masker was diametrically opposed to that of the target in polar coordinates (Fig. 1F).
