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In control experiments, rat brains were injected with PKH 26-labeled UCM cells that had been lysed by repeated sonic disruption.
In this set of experiments, rat E18 primary cortical neurons were cultured for 21 days in vitro and then transfected using Lipofectamine with the control or with miR-130a.
For all other experiments, rat phagocytes were used.
In this set of experiments, rat brain slice organotypic culture methods were modified from those published previously [26], [27].
For single-cell cloning experiments, rat CDCs were first plated at an assumed density of 1 cell per well in a 96-well plate coated with fibronectin.
In another set of experiments, rat cortical neurons (7-10 DIV) treated with AAP 0.5 and 1 mM for 24 hours showed chromatin condensation and nuclear fragmentation, a feature of apoptosis, whereas only round blue nuclei were observed in vehicle-treated cells (Figure 2B).
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In those experiments, rats taught to feel helpless and anxious (by being exposed to a laboratory stressor) showed increased serotonin activity in their brains.
In a further set of experiments RAT-1 fibroblasts were investigated expressing Cypridina noctiluca luciferase (CLuc) driven by the promoter of the circadian clock gene Mma11[178].
For small-animal PET/CT/MR experiments, rats were anesthetized by isoflurane.
In lab experiments, rats can distinguish shapes and make extremely fine distinctions between textures.
For the knock-down experiments, rats under anesthesia were placed double cannulas into the ARC.
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