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In one set of experiments, plates were coated directly with CP.
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For microarray experiments, plated ES cells were diluted at 105 cell/ml in ES cell medium with or without LIF.
For inhibition experiments, plated macrophages were pretreated with inhibitor at concentrations specified for 30 minutes before addition of ICs.
On the basis of the cell counting experiments, plating density was modified for different glucose medium to give similar cell densities at the time of assay.
On the basis of the cell counting experiments, plating density was modified for different glucose media to give similar cell densities at the time of assay.
We next manually picked 300 iPSC clones from the experiment plates at 8 dpt in order to catch pFind1-facilitated colonies.
In contrast, iPSC colonies appeared a few days earlier in the experiment plates that were transfected with OKS, pFind1 and pCAG-PBase.
The worms were transferred on the experiment plates 10 minutes before the start of recording to cut off the first minutes during which the worm may have responded to perturbations that could have occurred during transfer.
In a second experiment, plates were placed in containers, exposed to AA or volatiles for 5 d, and then inoculated with the fungal mycelium plug.
From the other set of experiment plates, we collected five fruiting bodies that had a D. discoideum phenotype and five fruiting bodies that had a D. purpureum phenotype.
In a third experiment, plates containing PDA medium were exposed to AA or volatiles for 24, 48, or 120 h, prior to inoculation.
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