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Our simulator capabilities as decision making tool are strongly amplified if Design of Experiment (DOE) and Analysis of Variance (ANOVA) are respectively used for experiments planning and simulation results analysis.
Authors' contribution: experiments planning and manuscript drafting: MA, GA and GM; cell culture and animal treatment: CG, EA and RM; cardiac contractility studies: EB and GA; biochemical parameters detection, western blot and PCR: MA and RM.
Experiments' planning was designed using Taguchi method to determine the influence of four parameters (transmembrane pressure, feed flow rate to the UF module, destabilization temperature, and coagulant salt molar concentration) on a response factor, with three levels for each of them.
AK has been involved in the experiments planning, the drafting of the manuscript and for revising it critically for content and general supervision of the project.
AMV, AC, KRL and MH has been involved in the experiments planning, the drafting of the manuscript and for revising it critically for content and general supervision of the project.
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