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Tarca, A. L., Romero, R. & Draghici, S. Analysis of microarray experiments of gene expression profiling.
Here, we critically evaluated the accuracy of flux balance analysis in two cancer types, clear cell renal cell carcinoma (ccRCC) and prostate adenocarcinoma, by comparison with large-scale experiments of gene essentiality in vitro.
Analysis of variance (one-way ANOVA) and multiple comparisons of differences between treatments (Duncan's multiple range test, p < 0.05, 0.01) were performed using SPSS for Windows version 11.5 (SPSS Inc .. XP performed the experiments of gene cloning and phenotype assays of transgenic plants.
We compared our theoretical predictions with the existing experiments of gene targeting using ODN in the literature.
All the experiments of gene expression profiling were authorized to be carried out by CapitalBio Corporation (Beijing, China).
Verification experiments of gene array findings included both real-time PCR analysis and in vitro metabolic tests.
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Furthermore, we performed, at the end of every experiment of gene quantification, a determination of the melting point of the amplified fragments, to ensure that only a single fragment of DNA is amplified in each tube.
Gene silencing experiments of reelin gene or its intracellular effector Dab-1 confirmed the implication of Reelin signaling as a survival factor for the limb undifferentiated mesoderm.
In our experiments, levels of gene expression were normalized using glyceraldehyde 3-phosphate dehydrogenase and β-actin.
The difference (ΔCt) between the average (three experiments) of the gene of interest (EGFR or HER2) and the housekeeping gene (GAPDH) was calculated using the software Microsoft Excel.
Jin performed the experiments of Toll gene expressions.
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