Further experiments are necessary for understanding the sequence dependence of thermodynamics for the stem 1−loop 3 region and for tertiary interactions.
A lung xenograft model had been generated previously and no additional animal experiments were necessary for the current work (Liu and Tsao, 1993).
Experiment is necessary for validating the electro-hydraulic camless variable valves actuation system design.
If the pho1 + promoter sequence bound by Pho7-TAP in the ChIP-Seq experiment is necessary for activation of pho1 + transcription during Pi-starvation, then deletion of this region should result in a loss of yfp + expression during Pi-starvation.
Therefore, the second experiment was necessary for a better understanding of the processes involved in haptic size perception with availability of weight information.
For long-term experiments, it is necessary for miRNA expression to be sustained.
Twenty to 30 larvae were transferred into a new vial amply supplemented with live yeast culture (high protein diet) 12 24 h before starvation to reduce crowding and make sure that all animals were well-fed at the onset of the starvation experiments, which is necessary for a proper autophagic response.
From a practical standpoint, especially, reasonable experiment design is necessary for in vitro experiments, as described in references [ 9, 38].
While this result might seem obvious from the fact that pitch is hard-coded in the cochlea, and flicker is encoded by low level, dedicated mechanisms, these experiments were necessary to directly address, for the first time, the time-perception-as-movie-camera framework.
