TEM micrographs were prepared from all the three experiments harvested after 4, 5 and 6 weeks and always showed identical results.
conceived the project, generated the D124 mutation, performed breeding experiments, harvested germ cell material, performed cell-sorting experiments, and conducted data analysis.
For attached-matrix experiments, harvested FPCLs were treated as described above except that they were not mechanically released from the sides of the dishes throughout the 5 day incubation period.
In a previous experiment harvested 3 days following siRNA transfection, this result was reversed and repression of FZD7 was only apparent with the FZD7-1 siRNA.
Since plants from N and P experiments were harvested at a later growth stage and were grown in different chambers than in the low temperature experiment, this component could be influenced by developmental differences.
All growth experiments were harvested every other day (Fig. 7) and showed a mean productivity of 16.1 g/m2/day (SD ±1.7) in 2013 and 15.4 g/m2/day (SD ±4.7) in 2014.
Independent replicate experiments were harvested for further analysis.
We waited three days after transfection of the siRNAs, while in the shRNA experiments we harvested two days after induction.
Neurosphere passage was done every 7 10 days, and the cells used for experiments were harvested after passage 2 3.
The leaves from plants used in the olfactometer experiments were harvested and flash-frozen in liquid nitrogen.
