One mutant identified as tolerant under both PEG and pot-screening experiments for enhanced tolerance to water stress, named as 'enhanced water stress tolerant1' (ewst1), and the WT were grown in three rows each in the research field of IARI, New Delhi at a spacing of 20 cm between rows and 15 cm between plants within a row following recommended agronomic practices.
We consider the method of designed experiments for statistical tolerance analysis, and study the impact of experimental error on its results.
After eight hours of incubation, cells were harvested for tolerance experiments.
Results from large selection experiments for several abiotic stress tolerances in rice (unpublished data) provide strong evidence in support of this prediction.
To investigate whether these enriched dnaQ mutants were the optimal that could always be enriched in independent evolution processes, we performed parallel experiments for acquiring kanamycin resistance, n-butanol tolerance, and acetate tolerance.
The performance of agronomic traits under a similar condition to the experiments for both salinity and drought tolerance has been reported in a previous publication [ 43] and among these different agronomic traits, awn length (AL) showed co-segregation with uzu gene [ 54].
In the afternoon and evening, Israeli teenagers gather there for their own experiments in tolerance -- checking out the Arab water-pipes and flavored tobacco peddled by Palestinian vendors, riffling through the Indian rugs and bedspreads brought back by former soldiers who vacationed abroad after finishing military service.
In the DT1 experiment, G. herbaceum accessions were subjected to mannitol stress in the screen for tolerance to osmotic stress.
Independent T3 homozygous transformants CdICE1-5 and CdICE1-8 were used for further experiments in freezing tolerance.
In our experiments, the mass tolerance for decoys was set at 20 ppm, maximum missed cleavage number was set to 2, peptide variation was set at 200 Da and the number of decoys per target was set to 20.
