Sentence examples for experiments for quantification from inspiring English sources
Particular emphasis is placed on optimizing experiments for quantification of GABA and glutamate.
The probabilistic analysis uses sets of statistically similar microstructure samples to directly mimic relevant experiments for quantification of statistical variations of material behavior due to inherent material heterogeneities.
Since the hybridization conditions may slightly vary between experiments, for quantification of the mean value for several independent experiments, the control value of each experiment was considered 100% and the experimental values were calculated accordingly.
In order to further validate our FACS-based method to quantify mitochondrial mass, we performed Western blot analyses of the mitochondrial complex IV subunit I, to quantify mitochondrial mass and to validate our FACS-based experiments for quantification by MitroTracker green staining (Invitrogen).
We performed 9 13 independent experiments for quantification.
Unsuppressed water signal was acquired at the end of the experiments for quantification of metabolites (eight averages).
The results of the quantifications are presented as means and standard error (SE) (n = 60 from 3 independent experiments for each quantification analysis).
Experiments for mRNA quantification as well as for GAPDH (as a housekeeping gene) were performed in Caco2/15 cells using the UNO II thermocycler (Biometra) as reported previously [35], [40].
In all experiments for protein quantification, each sample was hydrolyzed and analyzed three times, and the standard deviation of the means was calculated as (Σ(experimental value − average value)/2)1/2.
An external standard substance, trimethylsilyl propionic-2,2,3,3,-d4 acid ([TMSP] 20 and 50 mM in heavy water) in a thin sealed glass capillary was placed into the NMR tubes during all experiments for metabolite quantification and as a H chemical shift reference (at 0 ppm).
After the H and P qNMR experiments for the quantification of phosphonoacetic acid proved the independence of the received purity value from the nucleus, triphenyl phosphate could be quantified by H qNMR. Direct application of benzoic acid as standard reference material in proton NMR was not feasible due to the overlapping of the signals in the aromatic region.
