Sentence examples for experiments for protein from inspiring English sources

These siRNAs were, therefore, chosen in all further experiments for protein and cell proliferation studies.

In all experiments for protein quantification, each sample was hydrolyzed and analyzed three times, and the standard deviation of the means was calculated as (Σ(experimental value − average value)/2)1/2.

Similar to the experiments for protein localization prediction, we also conduct experiments to examine the effect of the proposed EGM-SMCC method (integrating multiple latent graphs) for enhancing the prediction performance against the GM-SMCC method using a single latent graph.

In all experiments, for protein preparation, 100 μl of cold RIPA lysis buffer [50 mM Tris-acetate, 150 mM NaCl, 1% (v/v) NP-40, 0.1% (w/v) SDS, pH 7.4] and freshly mixed protease inhibitor cocktail (1 mM PMSF, 20 μg/ml benzamidine, 10 μM leupeptin and 1 μM pepstatin) was added to 10 mg of dissected brain tissue or 1×10 cells.

A nano LC/MS system consisting of a Surveyor HPLC system (Thermo Scientific, Waltham, MA, USA) and ESI-quadruple IT MS (LCQ Deca XP-Plus, Thermo Scientific, Waltham, MA) equipped with a nano-ESI source was used to perform the MS/MS experiments for protein identification.

A study conducted recently suggested that multiple enzymatic digestions facilitated the detection of overlapping but distinct sets of proteins and provided a significant increase in the number of protein identifications in a complex proteomic sample [ 17]. Figure 1A shows the contribution of the two protease digestion approach, which was used in present experiments for protein identification.

When several months later the actual structures of these proteins were revealed, the predictions were found to be considerably more accurate than any previous predictions made in the 20 years of CASP experiments for proteins over 100 amino acids that lack homologs of known structure.

This is the problem we consider in this paper: given the results of a set of AP-MS experiments, filtered for protein misidentifications and contaminants, how can we distinguish direct (physical) interactions from indirect interactions?

Given the number of proteins with unknown functions, conducting biochemical and genetic experiments for each protein individually is unrealistic.

To quantify the extent of enrichment of the LC3 fusion proteins in the nucleus, we sampled the average fluorescence intensity of GFP within regions of interest placed within the cytoplasm and nucleus from confocal sections and calculated the nuclear/cytoplasmic ratio for 40 60 cells across 4 5 independent experiments for each protein (Figure 1B).

There were five assay experiments for this protein.