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They are derived mainly from four sources: high-throughput experiments for interaction detection (yeast two-hybrid, affinity purification followed by mass spectrometry, whole genome expression, literature mining, and genomic context).
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Other experiments for interactions were performed in the same procedure.
The evaluation of spoken dialog systems still relies on subjective interaction experiments for quantifying interaction behavior and user-perceived quality.
D-E.P. designed and performed most of the experiments, analysed data and contributed to writing the paper; R.L. performed initial experiments for LATS1 CDK2 interaction, γH2AX analysis, pBRCA2 western blots and I-SceI assay; I.P. transferred the DNA fibre technology; K.S.Y. performed initial immunoprecipitation experiments for RASSF1A effect on LATS1 CDK2 interaction.
These results may also be applicable in the laboratory experiments for laser plasma interaction, where dust acoustic shocks may be observed in the presence of dust grains and superthermal particles.
Firstly, we have carried out SPR binding experiments for the interaction of wild-type Norrin with Lrp6, and we have added these data to the manuscript.
Third, in our previous laboratory experiments testing for interactions of space, valence, and handedness [5], the temporal/numerical order of good and bad responses were counterbalanced, and the predicted interactions were found to be independent of order.
We further verify that a number of the reoriented residues were identified in mutational experiments as important for interaction with SRC-1, GRIP, TAFs co-activators and VDR-RXR heterodimerization.
140b Hydrogen/deuterium exchange experiments provided evidence for interaction between PqqE and PqqD, as the presence of PqqE provided partial protection of PqqD to H/D exchange.
Finally, we divide the number of reads of the experiment for each interaction by the expected value given by the fit at the genomic distance of the interaction.
The numerical model is verified by a flume channel experiment for the interaction mechanism between air and water.
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