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They are skillfully rendered what-if experiments for figuring out where the language of the original composer has led to now.
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Jianbo Yang conceived and developed experiments for Figures 1– 6, 8 and 10.
Rafiquel Sarker conceived and developed experiments for Figures 7, 9 and 11.
Experiments for Figure 3 were performed in 24-well plates with wells filled with 1.5 ml LB 1.5% agar each.
Nazan Saner and Kirsty Mustard made the FLAG-tagged CaMKKβ construct and performed pilot experiments for Figure 5. Grahame Hardie directed the project and wrote the first draft of the manuscript.
The following combinations were used in fluorescence experiments: For Figure 1, guinea pig anti-CGRP primary antibody, goat anti-guinea pig IgG secondary antibody, with fluorescein; followed by rabbit anti-Na,K-ATPase alpha-2 primary antibody (1 500), goat anti-rabbit IgG secondary antibody, with Texas red.
(A ) Schematic representation of pulse-chase experiment for Figure 3B.
The purpose of the experiment for Figure 4 is to test whether activation of D1 receptors is involved in fear reinstatement and its associated changes.
The authors should specify the number of independent experiments for each figure and estimate the variation between experiments.
We thank Martine Carreras for her precious help in the experiments for supplementary figures.
Consequently, we observed overlapping between the HC- and the soil experiments for 7C (Figure 5g).
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