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We performed two independent experiments for expression profiling.
In our experiments for expression in COS-1 cells, we found that phosphorylation of serine residues ("alkaline resistant phosphorylation") is more prominent in ATP7B than in ATP7A.
RT-PCR experiments for expression of adams 12, 13, and 19 were carried out using total RNA purified from X. tropicalis embryos at different developmental stages.
EGM performed statistical analysis of data, AL helped IHC staining/scoring, AAH generated/provided RIPK1-related molecular tools/models, RMDP and DF performed experiments for expression of recombinant I2PP2A/SET, DP and RVS helped design experiments, and BO conceived/designed experiments, analysed data, and wrote the manuscript.
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A slight discrepancy of 6% between the model and experiment for expression of the human ND2 gene might be explained by a similar bias in estimating half-lives [ 39] (Table 2).
We did not perform similar experiments for TNFR2 expression, since this receptor is already constitutively expressed on all activated T cells.
Table 2 shows trait means for the White Leghorns used in experiment 2 for expression profiling of candidate genes selected from experiment 1.
The siRNA-mediated interference experiments for eEF1γ expression were performed by transfecting SMART pool-specific or non-specific control pool double-stranded RNA oligonucleotides (Dharmacon) using Lipofectamine 2000.
Control experiments for pFAK expression have been previously described [ 18].
Experiments for gene expression analysis included the fifth condition of synoviocytes cultured alone.
CHL performed oligonucleotide microarray experiments for gene expression profile and helped in drafting manuscript.
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