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For example, the in-orbit ESA-facility EXPOSE on the International Space Station ISS can only accommodate a limited number of experiments for exposure to the space parameters high vacuum, intense radiation of galactic and solar origin and microgravity.
The stability of tagRFP was sufficient during our long-term WFMP-FLIM experiments for exposure times up to 10 h although a more photostable mutant has been recently synthesized (tagRFP-T) [38].
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We first performed a preliminary experiment for exposure to the epimutagen mixture, either throughout the culture period (both stem and differentiation periods, similar to condition III) or for 10 days following induction of EB differentiation.
Once the roots reached 0.2 cm in length, they were used for experiments of exposure to FA (Sigma, St . Louis MO, USA) under sterile conditions in the same Petri dish.
Three coated concrete specimens with epoxy, polyurethane, and chlorinated rubber coatings were fabricated and subjected to natural outdoor experiments of exposure degradation for one year.
Percentage changes in GFP fluorescence were calculated from the average maximum GFP intensity measured in each experiment after normalization for exposure time, if necessary.
Given the dilemma surrounding human experimentation, in 1998 the Clinton administration placed a moratorium on the EPA reviewing human experiments for setting permitted exposure levels.
Fertilized and normally developing embryos were selected under a stereomicroscope (×8 to × 50) at 4 h post-fertilization (hpf) (i.e., the sphere stage of the blastula period) and used for exposure experiments.
The prepared soil was kept at 4°C, and was used for exposure experiments within a week.
Briefly, wDE for exposure experiments was generated using a Yanmar diesel generator using low-sulfur diesel fuel (32 ppm).
Fresh diatom cultures were inoculated at a start cell density of 0.5 × 106 cells/mL, incubated for 24 h and divided into different flasks for exposure experiments.
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