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Small interfering RNA (siRNA) (Qiagen, sequences in Table S1, The control siRNA and the SOCS2 siRNAs were tested in control experiments for effects on mRNA expression of IFNβ and IFNα1 measured by Real-time PCR.
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3.To do so, we parameterized a density-dependent population projection matrix model for Piriqueta caroliniana, a widespread sandhill plant that often occurs at low density, using data from a demographic study of natural populations and from field experiments testing for effects of density on fecundity, growth and survival.
Additional file 1: Details of three-way ANOVA of factorial design experiment for effect of cell line and growth environment.
Experiments for studying effects of exogenous application of different chemicals such as AsA involve spray of aqueous solutions and any improvement in growth and salt tolerance by such treatments may be due to water rather than the chemical.
The second experiment tested for effects of wording differences.
We have reproduced these results with independent RNAse protection assays (two experiments for the effect of each mutant and three experiments for the maternal effect).
The qRT-PCR experiments for the effect of Δ133p53 on p53-inducible genes were replicated in two independent fibroblast strains BJ (Figure 3) and CRL-2097 (Supplementary Figure S2).
If these species are amenable to laboratory culture, this may allow for particularly powerful, controlled experiments that test for effects of resource availability on acquisition and allocation and how such patterns have changed coincident with nutrient change.
The spending panel delivered its most decisive blow to Brookhaven National Lab's Rare Symmetry Violating Processes (RSVP), a high-energy physics experiment to look for effects beyond the Standard Model.
Because the design does not require some needy individuals being assigned to a no-treatment group, it has ethical advantages over experiments for assessing treatment effects.
In this study, numerical mechanical tests and digital samples are proposed as an alternative to uniaxial strain experiments for investigating the effects of compaction.
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