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Based on recent experiments, current approaches for designing ECB connections are examined, and a new strength characterization method is presented.
During the implementation of the experiments, current knowledge in the field of voice recognition, voice recognition with additive background noise to determine the signal - noise ratio, language recognition, implementation of the ANFIS system to process the speech signal and in the field of filtering the noise from the speech signal using the ANFIS system, was used.
In these experiments, current steps were used instead of current ramps.
For EC50 experiments, current changes were measured for a range of PBA concentrations (1 10 mM).
During our experiments, current pulse amplitude was kept constant, which lead to a slightly higher voltage pulse amplitude when the neurons were under the influence of carbachol.
In these experiments current was injected in one of the cells while the response to the current injection was recorded in the respective other cell [40].
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For these experiments, currents were filtered at 5 kHz and access resistance was compensated 80 90%.
For charge carrier experiments, currents were measured in modified KD50 containing either 50 mM KOH, RbCl, or CsCl.
For all experiments, currents were filtered at 1 kHz, digitized at 20 kHz, and analysed with pCLAMP9.2 and ORIGIN6.0 software.
For this validation experiment, current methods for location and scale normalisations of log-ratio values were used [ 14- 16].
Over the time course of the experiment, currents expressed by scn8aa alone or scn8aa coexpressed with Scn1b inactivated fully.
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