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At the end of the experiments, cultures were harvested.
For experiments, cultures were washed to remove non-adherent cells and fresh medium was added.
In some experiments, cultures were used for immunohistochemistry after patch-clamp recordings.
For chronological ageing experiments, cultures were grown in flasks with vigorous shaking and processed as previously described [37], [79].
In certain experiments, cultures were treated with bafilomycin A1 (100 nM; Sigma Chemical) for 2 h prior to autophagy assessment.
In all our Synechocystis experiments, cultures were maintained at steady state growth for a minimum of 7 generations prior to harvesting.
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After cell culture experiments, culture media were removed and cells were washed two times with PBS at 37°C.
24 hours before experiments, culturing DMEM was replaced with fresh FBS 10% DMEM without antibiotics.
For some control experiments, culture inserts (Ibidi, Munich, Germany) were used to create a cell-free gap.
Twenty four hours before starting the experiments, culture medium was replaced by a basal VSMC medium without fetal calf serum.
For permeability experiments, culture inserts were placed to 24-well plates (Greiner, Austria) containing 530 µl Ringer-Hepes buffer.
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