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For colocalization experiments, constructs were co-injected into N2 worms.
In separate experiments, constructs were subjected to dynamic compression or remained unstrained within a bioreactor system.
For the Hes1 reporter experiments constructs of pBI-TRE-cId2/cId3/mFoxc2 were used following excision of the GFP cassette.
For RNA interference experiments, constructs were generated in pSUPER.retro.puro (OligoEngine, Seattle, WA, USA) using specific oligonucleotides (Invitrogen) targeting SMN sequence as described.
In separate experiments, constructs were cultured under free-swelling conditions or subjected to dynamic compression with CNP, interleukin-1β (IL-1β), the Npr2 antagonist P19 or the Npr3 agonist cANF.
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If experiential learning activities support engagement and deeper student learning, student-owned experiments constructed "from the ground up" might have benefits that exceed pre-designed classroom experiences.
It is a unit of statistical tools for designing experiments, constructing models, assessing the effects of factors, and exploring optimum conditions of factors under study for desirable responses (de-Coninck et al. 2000).
AP helped design yeast experiments, constructed strains and performed viability analysis and helped write the manuscript.
JMG conceived the study, designed experiments, constructed yeast strains, analysed data and wrote the paper.
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